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Proteomic identification of rainbow trout seminal plasma proteins
Author(s) -
Nynca Joanna,
Arnold Georg J.,
Fröhlich Thomas,
Otte Kathrin,
Flenkenthaler Florian,
Ciereszko Andrzej
Publication year - 2014
Publication title -
proteomics
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300267
Subject(s) - rainbow trout , hemopexin , blood proteins , proteome , albumin , transferrin , proteomics , trout , acute phase protein , biology , semen , haptoglobin , fractionation , chemistry , biochemistry , inflammation , fish <actinopterygii> , immunology , chromatography , anatomy , fishery , enzyme , heme , gene
In the study, the combination of protein fractionation by 1DE and HPLC-ESI-MS/MS was used to characterize the rainbow trout seminal plasma proteome. Our results led to the creation of a catalogue of rainbow trout seminal plasma proteins (152 proteins) and significantly contributed to the current knowledge regarding the protein composition of fish seminal plasma. The major proteins of rainbow trout seminal plasma, such as transferrin, apolipoproteins, complement C3, serum albumin, and hemopexin-, alpha-1-antiproteinase-, and precerebellin-like protein, were recognized as acute-phase proteins (proteins that plasma concentration changes in response to inflammation). This study provides the basis for further functional studies of fish seminal plasma proteins, as well as for the identification of novel biomarkers for sperm quality. The MS data have been deposited in the ProteomeXchange with identifier PXD000306 (http://proteomecentral.proteomexchange.org/dataset/PXD000306).