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Proteomics in investigation of cancer metastasis: Functional and clinical consequences and methodological challenges
Author(s) -
Maryáš Josef,
Faktor Jakub,
Dvořáková Monika,
Struhárová Iva,
Grell Peter,
Bouchal Pavel
Publication year - 2014
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300264
Subject(s) - proteomics , interactome , proteome , computational biology , metastasis , cancer , disease , identification (biology) , bioinformatics , biology , mechanism (biology) , quantitative proteomics , medicine , pathology , biochemistry , philosophy , genetics , botany , epistemology , gene
Metastases are responsible for most of the cases of death in patients with solid tumors. There is thus an urgent clinical need of better understanding the exact molecular mechanisms and finding novel therapeutics targets and biomarkers of metastatic disease of various tumors. Metastases are formed in a complicated biological process called metastatic cascade. Up to now, proteomics has enabled the identification of number of metastasis‐associated proteins and potential biomarkers in cancer tissues, microdissected cells, model systems, and secretomes. Expression profiles and biological role of key proteins were confirmed in verification and functional experiments. This communication reviews these observations and analyses the methodological aspects of the proteomics approaches used. Moreover, it reviews contribution of current proteomics in the field of functional characterization and interactome analysis of proteins involved in various events in metastatic cascade. It is evident that ongoing technical progress will further increase proteome coverage and sample capacity of proteomics technologies, giving complex answers to clinical and functional questions asked.