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Exploring intercellular signaling by proteomic approaches
Author(s) -
Tian Ruijun
Publication year - 2014
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300259
Subject(s) - proteomics , biology , microbiology and biotechnology , cell signaling , intracellular , signal transduction , context (archaeology) , quantitative proteomics , cell , computational biology , biochemistry , paleontology , gene
Cells live in a close social context by having mutual communication with their local microenvironment. This complex intercellular communication activates dynamic signaling pathways and regulates specific cell fate. MS ‐based proteomics has been approved to be inevitable for characterizing dynamic protein expression and PTM s on a global scale. However, because of technical difficulties for targeting membrane receptors and secreted proteins, especially in a physiologically relevant manner, systematic characterization of intercellular signaling by MS ‐based proteomics has largely lagged behind. Here, I will review the latest proteomics technology development and its application to characterizing different modes of intercellular communication including indirect and direct cell–cell communication, and protein translocalization. I will discuss how MS ‐based proteomics has been applied for systems‐level profiling intercellular signaling in defined biological contexts including tumor microenvironment, bacteria/virus–host cell interaction, immune cell interaction, and stem cell niche.