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Proteomics analysis of UV ‐irradiated L onicera japonica T hunb. with bioactive metabolites enhancement
Author(s) -
Zhang Lin,
Li Ximin,
Zheng Wen,
Fu Zhirong,
Li Wenting,
Ma Luyu,
Li Ke,
Sun Lianli,
Tian Jingkui
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300212
Subject(s) - biochemistry , proteomics , chemistry , metabolism , secondary metabolism , metabolic pathway , bioactive compound , biosynthesis , atp synthase , enzyme , lipid metabolism , gene
A previous study showed that the contents of caffeoylquinic acids and iridoids, the major bioactive components in the postharvest L onicera japonica T hunb., were induced by enhanced ultraviolet ( UV )‐ A or UV ‐ B irradiation. To clarify the UV ‐responsive key enzymes in the bioactive metabolites biosynthetic pathway and the related plant defense mechanism in L. japonica , 2 DE in combination with MALDI ‐ TOF / TOF MS was employed. Seventy‐five out of 196 differential proteins were positively identified. Based on the functions, these proteins were grouped into nine categories, covering a wide range of molecular processes including the secondary metabolites (caffeoylquinic acids and iridoids) biosynthetic‐related proteins, photosynthesis, carbohydrate and energy metabolism, stress, DNA , transport‐related proteins, lipid metabolism, amino acid metabolism, cell wall. Of note is the increasing expression of 1‐deoxy‐ d ‐xylulose 5‐phosphate reductoisomerase and 5‐enol‐pyruvylshikimate‐phosphate synthase, which was crucial to supply more precursor for the secondary metabolites including caffeoylquinic acids and iridoids. Thus, this study provides both the clues at the protein level for the increase of the two bioactive components upon UV irradiation and the profile of UV ‐responsive proteins in L . japonica .