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Beyond antibodies: New affinity reagents to unlock the proteome
Author(s) -
Lollo Bridget,
Steele Fintan,
Gold Larry
Publication year - 2014
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300187
Subject(s) - aptamer , proteome , computational biology , reagent , biomarker , biomarker discovery , antibody , chemistry , proteomics , combinatorial chemistry , computer science , biology , biochemistry , microbiology and biotechnology , immunology , gene
Antibodies have been the workhorse reagents of protein capture and quantification since their 1959 debut in the RIA s developed by Yalow and Berson. However, there are technical challenges to the use of antibodies in highly multiplexed arrays aimed at measuring hundreds or even thousands of proteins at one time. We describe here a recently developed class of synthetic protein‐binding reagents (slow off‐rate modified aptamer). We discuss the chemical makeup and protein binding specifications of slow off‐rate modified aptamer reagents, compare them to traditional aptamers and antibodies, briefly describe the novel proteomic assay that takes advantage of their unique properties, and provide several examples of their multiple applications to biomarker discovery and validation across a range of biomedical science questions.