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Surface proteomic analysis of differentiated versus stem‐like osteosarcoma human cells
Author(s) -
Gemei Marica,
Corbo Claudia,
D'Alessio Francesca,
Di Noto Rosa,
Vento Renza,
Del Vecchio Luigi
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300170
Subject(s) - stem cell , cancer stem cell , biology , microbiology and biotechnology , osteosarcoma , mapk/erk pathway , cell culture , kinase , cell , cellular differentiation , cancer research , chemistry , biochemistry , genetics , gene
Cancer stem cell characterization represents a breakthrough in cancer research. Despite evidence showing the existence and the role of cancer stem cells in osteosarcoma ( OS ) onset and progression, little is known about their specific surface phenotype. To address this issue, we carried out a cytometric analysis with an antibody‐array comprising 245 membrane proteins comparing the stem and differentiated OS cells. As experimental model, we chose the stem‐like cell line 3aminobenzamide‐ OS and its parental, differentiated, cell line MG 63. We identified 50 differentially expressed, 23 homogeneously expressed, and 172 not expressed proteins in the two cell line models, thus defining a surface protein signature specific for each of them. Furthermore, we selected ERK 1/2 (p44/42 mitogen‐activated protein kinases) as a potential pathway correlated with processes that characterize tumorigenic potential and stemness of 3aminobenzamide‐ OS cells.