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Proteomic approaches to sexual development mediated by antheridiogen in the fern Blechnum spicant L
Author(s) -
Valledor Luis,
Menéndez Virginia,
Canal María Jesús,
Revilla Angeles,
Fernández Helena
Publication year - 2014
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300166
Subject(s) - gametophyte , fern , biology , botany , spore , proteomics , germination , biochemistry , gene , pollen
This report is intended to compare proteomic profiles between female and male gametophytes of Blechnum spicant L. For this purpose, female gametophytes were obtained from spores germinated on Murashige and Skoog medium (1962), and the male ones by culturing 30‐day‐old gametophytes on the same medium supplemented with extract derived from mature gametophytes, which exhibits anteridiogen effect. Proteins were extracted using the phenolic method, and after two‐dimensional gel electrophoresis, 581 spots were detected in Coomassie stained gels within the 3–11 pH range and 10–100 kDa M r ranges. Quantitative and qualitative differences in spot intensity between both types of gametophytes were found. The induction of male gametophytes by antheridiogens was correlated to a global increase in stress and defence‐related pathways, which affect flavonoid signaling and cell division, while reducing protein biosynthesis, photosynthesis, and other energy‐related pathways, reflecting how stressed are the gametophytes during the induction process. This is the first proteomic study that examines proteins associated to sexual development in the gametophyte of the fern B. spìcant .