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Two‐dimensional proteome reference map of P rototheca zopfii revealed reduced metabolism and enhanced signal transduction as adaptation to an infectious life style
Author(s) -
Murugaiyan Jayaseelan,
Weise Christoph,
Bergen Martin,
Roesler Uwe
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300037
Subject(s) - biology , genotype , proteome , gene , microbiology and biotechnology , genetics
Biochemical, serological, and genetic analyses have identified two genotypes of P rototheca zopfii , a unicellular microalga belonging to the family C hlorellaceae . The P . zopfii genotype 1, abundantly present in cow barns and environment, remains nonpathogenic, while P . zopfii genotype 2 has been isolated from cows with bovine mastitis. The present study was carried out to identify the protein expression level difference between the pathogenic and nonpathogenic strains of P . zopfii . A total of 782 protein spots were observed on the 2D fluorescence difference gel electrophoresis (2D DIGE) gels among which 63 and 44 proteins were identified to be overexpressed in genotypes 1 and 2, respectively. The limited number of protein entries specific for P rototheca in public repositories resulted mainly in the identification of proteins described in other algae, microorganisms, or plants. Gene ontology (GO) analysis indicated reduced carbohydrate metabolism in genotype 1, while genotype 2 displayed enhanced DNA binding, kinase activity, and signal transduction. These effects point to metabolic and signaling adaptations in the pathogenic strain and provide insights into the evolution of otherwise highly similar strains. All MS data have been deposited in the ProteomeXchange with identifier PXD000126.