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Proteome analysis of functionally differentiated bovine ( B os indicus ) mammary epithelial cells isolated from milk
Author(s) -
Janjanam Jagadeesh,
Jamwal Manu,
Singh Surender,
Kumar Saravanan,
Panigrahi Aswini K.,
Hariprasad Gururao,
Jena Manoj K.,
Anand Vijay,
Kumar Sudarshan,
Kaushik Jai K.,
Dang Ajay K.,
Mukesh Manishi,
Mishra Bishnu P.,
Srinivasan Alagiri,
Reddy Vanga S.,
Mohanty Ashok K.
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300031
Subject(s) - lactation , proteome , biology , proteomics , blot , secretion , mammary gland , microbiology and biotechnology , biochemistry , gene , genetics , pregnancy , cancer , breast cancer
Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells ( MEC s) constitute the innermost layer of aveoli. They are milk‐secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins in the metabolically active MEC s during lactation or their functional role in the lactation process. In the present investigation, we have reported the proteome map of MEC s in lactating cows using 2 DE MALDI ‐ TOF / TOF MS and 1 D ‐ G el‐ LC ‐ MS / MS . MEC s were isolated from milk using immunomagnetic beads and confirmed by RT ‐ PCR and W estern blotting. The 1 D ‐ G el‐ LC ‐ MS / MS and 2 DE ‐ MS / MS based approaches led to identification of 431 and 134 proteins, respectively, with a total of 497 unique proteins. Proteins identified in this study were clustered into functional groups using bioinformatics tools. Pathway analysis of the identified proteins revealed 28 pathways ( p < 0.05) providing evidence for involvement of various proteins in lactation function. This study further provides experimental evidence for the presence of many proteins that have been predicted in annotated bovine genome. The data generated further provide a set of bovine MEC ‐specific proteins that will help the researchers to understand the molecular events taking place during lactation.