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Surfaceomics and surface‐enhanced R aman spectroscopy of environmental microbes: Matching cofactors with redox‐active surface proteins
Author(s) -
Carlson Hans K.,
Iavarone Anthony T.,
Coates John D.
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201300010
Subject(s) - redox , cofactor , chemistry , cell , trypsin , biochemistry , proteomics , bacteria , biophysics , biology , enzyme , inorganic chemistry , genetics , gene
Trypsin shaving is a targeted proteomic method for identifying cell‐surface exposed proteins on bacterial cells. For the identification of redox‐active cell‐surface proteins, trypsin‐shaving datasets can be matched with surface‐enhanced R aman spectra of intact cells to identify the cofactors associated with the cell‐surface proteins. Together, these approaches could help resolve questions about the presence of cell‐surface electron transport components in environmental microorganisms, especially microbes that oxidize and reduce metals and metalloids as electron donors and acceptors.