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Antibody‐based profiling of cerebrospinal fluid within multiple sclerosis
Author(s) -
Häggmark Anna,
Byström Sanna,
Ayoglu Burcu,
Qundos Ulrika,
Uhlén Mathias,
Khademi Mohsen,
Olsson Tomas,
Schwenk Jochen M.,
Nilsson Peter
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200580
Subject(s) - cerebrospinal fluid , multiple sclerosis , antibody , immunology , chemistry , biology , chromatography , pathology , medicine
Antibody suspension bead arrays have proven to enable multiplexed and high‐throughput protein profiling in unfractionated plasma and serum samples through a direct labeling approach. We here describe the development and application of an assay for protein profiling of cerebrospinal fluid ( CSF ). While setting up the assay, systematic intensity differences between sample groups were observed that reflected inherent sample specific total protein amounts. Supplementing the labeling reaction with BSA and I g G diminished these differences without impairing the apparent sensitivity of the assay. We also assessed the effects of heat treatment on the analysis of CSF proteins and applied the assay to profile 43 selected proteins by 101 antibodies in 339 CSF samples from a multiple sclerosis ( MS ) cohort. Two proteins, GAP 43 and SERPINA3 were found to have a discriminating potential with altered intensity levels between sample groups. GAP 43 was detected at significantly lower levels in secondary progressive MS compared to early stages of MS and the control group of other neurological diseases. SERPINA3 instead was detected at higher levels in all MS patients compared to controls. The developed assay procedure now offers new possibilities for broad‐scale protein profiling of CSF within neurological disorders.