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Identification of host proteins modulated by the virulence factor AC 2 of T omato chlorotic mottle virus in N icotiana benthamiana
Author(s) -
Carmo Lilian Silveira Travassos,
Resende Renato Oliveira,
Silva Luciano Paulino,
Ribeiro Simone Graça,
Mehta Angela
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200547
Subject(s) - nicotiana benthamiana , biology , potato virus x , mottle , plant virus , tobacco mosaic virus , gene silencing , virology , potexvirus , virus , virulence , microbiology and biotechnology , rna , gene , genetics , coat protein
Tomato, one of the most important crops cultivated worldwide, has been severely affected by begomoviruses such as the T omato chlorotic mottle virus ( T o CM o V ). Virulence factor AC 2 is considered crucial for a successful virus–plant interaction and is known to act as a transcriptional activator and in some begomoviruses to function as an RNA silencing suppressor factor. However, the exact functions of the AC 2 protein of the begomovirus T o CM o V are not yet established. The aim of the present study was to identify differentially expressed proteins of the model plant N icotiana benthamiana in response to the expression of the AC 2 gene, isolated from T o CM o V . N . benthamiana plants were inoculated with A grobacterium tumefaciens containing the viral vector P otato virus X ( PVX ) and with the PVX ‐ AC 2 construction. 2 DE was performed and proteins were identified by MS . The results showed that the expression of T o CM o V AC 2 alters the levels of several host proteins, which are important for normal plant development, causing an imbalance in cellular homeostasis. This study highlights the effect of AC 2 in the modulation of plant defense processes by increasing the expression of several oxidative stress‐related and pathogenesis‐related proteins, as well as its role in modulating the proteome of the photosynthesis and energy production systems.