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Secretome analysis of the rice bacterium X anthomonas oryzae ( X oo ) using in vitro and in planta systems
Author(s) -
Wang Yiming,
Kim Sang Gon,
Wu Jingni,
Huh HyunHye,
Lee SuJi,
Rakwal Randeep,
Kumar Agrawal Ganesh,
Park ZeeYong,
Young Kang Kyu,
Kim Sun Tae
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200454
Subject(s) - xanthomonas oryzae , biology , secretory protein , microbiology and biotechnology , genetically modified rice , oryza sativa , proteomics , secretion , xanthomonas , pathogen , bacteria , effector , protease , gene , transgene , genetically modified crops , biochemistry , genetics , enzyme
Xanthomonas oryzae pv. oryzae ( X oo ) causes bacterial blight disease in rice, and that severely affects yield loss (upto 50%) of total rice production. Here, we report a proteomics investigation of X oo (compatible race K 3)‐secreted proteins, isolated from its in vitro culture and in planta infected rice leaves. 2 DE coupled with MALDI ‐ TOF ‐ MS and/or n LC ‐ ESI ‐ MS / MS approaches identified 139 protein spots (out of 153 differential spots), encoding 109 unique proteins. Identified proteins belonged to multiple biological and molecular functions. Metabolic and nutrient uptake proteins were common up to both in vitro and in planta secretomes. However, pathogenicity, protease/peptidase, and host defense‐related proteins were highly or specifically expressed during in planta infection. A good correlation was observed between protein and transcript abundances for nine proteins secreted in planta as per semiquantitative RT ‐ PCR analysis. Transgenic rice leaf sheath (carrying PBZ 1 promoter:: GFP cell death reporter), when used to express a few of the identified secretory proteins, showed a direct activation of cell death signaling, suggesting their involvement in pathogenicity related with secretion effectors. This work furthers our understanding of rice bacterial blight disease, and serves as a resource for possible translation in generating disease resistant rice plants for improved seed yield.