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Shotgun proteomic analysis of the Bombyx mori anterior silk gland: An insight into the biosynthetic fiber spinning process
Author(s) -
Yi Qiying,
Zhao Ping,
Wang Xin,
Zou Yong,
Zhong Xiaowu,
Wang Chen,
Xiang Zhonghuai,
Xia Qingyou
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200443
Subject(s) - bombyx mori , silk , shotgun , proteomics , shotgun proteomics , cuticle (hair) , microbiology and biotechnology , spinning , fiber , biology , function (biology) , biochemistry , chemistry , anatomy , gene , materials science , polymer chemistry , organic chemistry , composite material
The Bombyx mori anterior silk gland (ASG) is a natural fiber manipulator for the material provided by the middle and posterior silk glands. In view of the significant role of the ASG in the liquid–crystal spinning process, a shotgun proteomics approach was taken to study the relationship between the function of proteins in the silkworm ASG and the spinning mechanism. A total of 1132 proteins with 7647 unique peptides were identified in the ASG dataset including some involved in the cuticle, ion transportation, energy metabolism, and apoptosis. Two putative cuticle‐specific proteins were highly and specifically expressed in the ASG; therefore, the ASG dataset could provide clues for comprehensive understanding of the natural silk spinning mechanism in the silkworm. All MS data have been deposited in the ProteomeXchange with identifier PXD000090.