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Physiological and proteome study of sunflowers exposed to a polymetallic constraint
Author(s) -
Printz Bruno,
Sergeant Kjell,
Guignard Cedric,
Renaut Jenny,
Hausman JeanFrancois
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200400
Subject(s) - sunflower , helianthus annuus , biology , photosynthesis , phytoremediation , proteome , micronutrient , population , botany , nutrient , proteomics , agronomy , horticulture , chemistry , contamination , gene , ecology , biochemistry , demography , organic chemistry , sociology
The new energy requirements of the growing world population together with the actual ecological trend of phytoremediation have made challenging the cultivation of energetic crops on nonagricultural lands, such as those contaminated with trace elements. In this study, phenotypical characterization and biochemical analyses were combined to emphasize the global response of young sunflowers ( H elianthus annuus L .) grown in hydroponic media contaminated with different C d, N i, and Z n concentrations. Leaves and roots of sunflowers reaching the stage “2‐extended leaves” and exposed to different trace metal concentrations were harvested and analyzed by 2 D ‐ DIGE in order to study in depth the molecular responses of the young plants upon the polymetallic exposure. Proteomics confirmed the observed global reduction in growth and development. If photosynthetic light reactions and carbon metabolism were the most affected in leaves, in roots significant disruptions were observed in proteins involved in respiration, oxidative balance, protein and gene expression, and in the induction of programmed cell death. Elemental analyses of the plantlets indicated a profound impact of the treatment resulting in misbalance in essential micronutrients. Altogether, this study highlights the sensitivity of the sunflower to a polymetallic pollution and indicates that its use as a remediative tool of trace element polluted soils is limited.