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Proteome profiling of peripheral mononuclear cells from human blood
Author(s) -
Maccarrone Giuseppina,
Rewerts Christiane,
Lebar Maria,
Turck Christoph W.,
MartinsdeSouza Daniel
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200377
Subject(s) - proteome , peripheral blood mononuclear cell , proteomics , peripheral blood , biology , computational biology , label free quantification , immunology , bioinformatics , in vitro , gene , quantitative proteomics , biochemistry
Peripheral blood mononuclear cells ( MNC s) are accessible through blood collection and represent a useful source for investigations on disease mechanisms and treatment response. Aiming to build a reference proteome database, we generated three proteome data sets from MNC s using a combination of SDS ‐ PAGE and nanoflow LC‐MS. Experiments were performed in triplicates and 514 unique proteins were identified by at least two non‐redundant peptides with 95% confidence for all replicates. Identified proteins are associated with a range of dermatologic, inflammatory and neurological conditions as well as molecular processes, such as free radical scavenging and cellular growth and proliferation. Mapping the MNC proteome provides a valuable resource for studies on disease pathogenesis and the identification of therapeutic targets.