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Investigation of stable and transient protein–protein interactions: Past, present, and future
Author(s) -
Ngounou Wetie Armand G.,
Sokolowska Izabela,
Woods Alisa G.,
Roy Urmi,
Loo Joseph A.,
Darie Costel C.
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200328
Subject(s) - transient (computer programming) , focus (optics) , computational biology , proteomics , function (biology) , computer science , biology , bioinformatics , microbiology and biotechnology , biochemistry , physics , gene , optics , operating system
This article presents an overview of the literature and a review of recent advances in the analysis of stable and transient protein–protein interactions ( PPI s) with a focus on their function within cells, organs, and organisms. The significance of PTMs within the PPI s is also discussed. We focus on methods to study PPI s and methods of detecting PPI s, with particular emphasis on electrophoresis‐based and MS ‐based investigation of PPI s, including specific examples. The validation of PPI s is emphasized and the limitations of the current methods for studying stable and transient PPI s are discussed. Perspectives regarding PPI s, with focus on bioinformatics and transient PPI s are also provided.