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Identification and characterization of proteins isolated from microvesicles derived from human lung cancer pleural effusions
Author(s) -
Park Jung Ok,
Choi DoYoung,
Choi DongSic,
Kim Hee Joung,
Kang Jeong Won,
Jung Jae Hun,
Lee Jeong Hwa,
Kim Jayoung,
Freeman Michael R.,
Lee Kye Young,
Gho Yong Song,
Kim Kwang Pyo
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200323
Subject(s) - microvesicles , lung cancer , pleural effusion , cancer research , cancer , exosome , microvesicle , tumor progression , medicine , malignant pleural effusion , microrna , pathology , biology , gene , biochemistry
Microvesicles ( MV s, also known as exosomes, ectosomes, microparticles) are released by various cancer cells, including lung, colorectal, and prostate carcinoma cells. MV s released from tumor cells and other sources accumulate in the circulation and in pleural effusion. Although recent studies have shown that MV s play multiple roles in tumor progression, the potential pathological roles of MV in pleural effusion, and their protein composition, are still unknown. In this study, we report the first global proteomic analysis of highly purified MV s derived from human nonsmall cell lung cancer ( NSCLC ) pleural effusion. Using nano‐ LC – MS / MS following 1 D SDS ‐ PAGE separation, we identified a total of 912 MV proteins with high confidence. Three independent experiments on three patients showed that MV proteins from PE were distinct from MV obtained from other malignancies. Bioinformatics analyses of the MS data identified pathologically relevant proteins and potential diagnostic makers for NSCLC , including lung‐enriched surface antigens and proteins related to epidermal growth factor receptor signaling. These findings provide new insight into the diverse functions of MV s in cancer progression and will aid in the development of novel diagnostic tools for NSCLC .