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Tissue proteomics of the low‐molecular weight proteome using an integrated c LC ‐ ESI ‐ QTOFMS approach
Author(s) -
Alvarez MeiHwa Tanielle Bench,
Shah Dipti Jigar,
Thulin Craig D.,
Graves Steven W.
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200291
Subject(s) - proteome , proteomics , homogenization (climate) , liver tissue , biology , peptide , spleen , computational biology , biochemistry , chemistry , microbiology and biotechnology , immunology , endocrinology , gene , biodiversity , ecology
Analysis of the protein/peptide composition of tissue has provided meaningful insights into tissue biology and even disease mechanisms. However, little has been published regarding top down methods to investigate lower molecular weight ( MW ) (500–5000 D a) species in tissue. Here, we evaluate a tissue proteomics approach involving tissue homogenization followed by depletion of large proteins and then c LC ‐ MS (where c stands for capillary) analysis to interrogate the low MW /low abundance tissue proteome. In the development of this method, sheep heart, lung, liver, kidney, and spleen were surveyed to test our ability to observe tissue differences. After categorical tissue differences were demonstrated, a detailed study of this method's reproducibility was undertaken to determine whether or not it is suitable for analyzing more subtle differences in the abundance of small proteins and peptides. Our results suggest that this method should be useful in exploring the low MW proteome of tissues.