Premium
Identification of cell wall proteins in the flax ( L inum usitatissimum ) stem
Author(s) -
Day Arnaud,
Fénart Stéphane,
Neutelings Godfrey,
Hawkins Simon,
Rolando Christian,
Tokarski Caroline
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200257
Subject(s) - linum , proteome , biology , cell wall , identification (biology) , arabidopsis thaliana , arabidopsis , transcriptome , botany , proteomics , microbiology and biotechnology , computational biology , biochemistry , gene , gene expression , mutant
Sequential salt ( C a C l 2 , L i C l) extractions were used to obtain fractions enriched in cell wall proteins ( CWP s) from the stem of 60‐day‐old flax ( Linum usitatissimum ) plants. High‐resolution FT ‐ ICR MS analysis and the use of recently published genomic data allowed the identification of 11 912 peptides corresponding to a total of 1418 different proteins. Subcellular localization using T arget P , P redotar, and W o LF PSORT led to the identification of 152 putative flax CWP s that were classified into nine different functional classes previously established for Arabidopsis thaliana . Examination of different functional classes revealed the presence of a number of proteins known to be involved in, or potentially involved in cell‐wall metabolism in plants. The flax stem cell wall proteome was also compared with transcriptomic data previously obtained on comparable samples. This study represents a major contribution to the identification of CWP s in flax and will lead to a better understanding of cell wall biology in this species.