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Differential proteomic analysis in human cells subjected to ribosomal stress
Author(s) -
Caterino Marianna,
Corbo Claudia,
Imperlini Esther,
Armiraglio Marta,
Pavesi Elisa,
Aspesi Anna,
Loreni Fabrizio,
Dianzani Irma,
Ruoppolo Margherita
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200242
Subject(s) - ribosomal protein , biology , ribosomal rna , translation (biology) , proteomics , ribosome , microbiology and biotechnology , internal ribosome entry site , transfection , protein biosynthesis , computational biology , cytoskeleton , gene , messenger rna , cell , genetics , rna
The biochemical phenotype of cells affected by ribosomal stress has not yet been studied in detail. Here we report a comparative proteomic analysis of cell lines silenced for the RPS19 gene versus cell lines transfected with scramble shRNA cells performed using the DIGE technology integrated to bioinformatics tools. Importantly, to achieve the broadest possible understanding of the outcome, we carried out two independent DIGE experiments using two different pH ranges, thus, allowing the identification of 106 proteins. Our data revealed the deregulation of proteins involved in cytoskeleton reorganization, PTM s, and translation process. A subset (26.9%) of these proteins is translated from transcripts that include internal ribosome entry site motifs. This supports the hypothesis that during ribosomal stress translation of specific messenger RNA s is altered.