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Proteomic analysis revealed the altered tear protein profile in a rabbit model of S jögren's syndrome‐associated dry eye
Author(s) -
Zhou Lei,
Wei Ruihua,
Zhao Ping,
Koh Siew Kwan,
Beuerman Roger W.,
Ding Chuanqing
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200230
Subject(s) - tears , proteomics , antibody , lacrimal gland , pathology , albumin , proteome , microbiology and biotechnology , medicine , immunology , chemistry , biology , biochemistry , gene
Sjögren's syndrome ( SS ) is an autoimmune disease that results in pathological dryness of mouth and eye. The diagnosis of SS depends on both clinical evaluation and specific antibodies. The goal of this study was to use quantitative proteomics to investigate changes in tear proteins in a rabbit model of SS ‐associated dry eye, induced autoimmune dacryoadenitis ( IAD ). Proteomic analysis was performed by i TRAQ and nano LC ‐ MS / MS on tears collected from the ocular surface, and specific proteins were verified by high resolution MRM . It was found that in the tears of IAD rabbits at 2 and 4 weeks after induction, S 100 A 6, S 1 00 A 9, and serum albumin were upregulated, whereas serotransferrin ( TF ), prolactin‐inducible protein ( PIP ), polymeric immunoglobulin receptor (p I g R ), and I g gamma chain C region were downregulated. High resolution MRM with m TRAQ labeling verified the changes in S 100 A 6, TF , PIP , and p I g R . Our results indicated significant changes of tear proteins in IAD rabbits, suggesting these proteins could potentially be used as biomarkers for the diagnosis and prognosis of dry eye. Several of these proteins were also found in the tears of non‐ SS dry eye patients indicating a common basis of ocular surface pathology, however, p I g R appears to be unique to SS .