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Proteome variation among F ilifactor alocis strains
Author(s) -
Aruni A. Wilson,
Roy Francis,
Sandberg Lawrence,
Fletcher Hansel M.
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200211
Subject(s) - virulence , biology , porphyromonas gingivalis , proteome , microbiology and biotechnology , proteases , gene , virology , genetics , bacteria , enzyme , biochemistry
F ilifactor alocis , a G ram‐positive anaerobic rod, is now considered one of the marker organisms associated with periodontal disease. Although there was heterogeneity in its virulence potential, this bacterium was shown to have virulence properties that may enhance its ability to survive and persist in the periodontal pocket. To gain further insight into a possible mechanism(s) of pathogenesis, the proteome of F . alocis strains was evaluated. Proteins including several proteases, neutrophil‐activating protein A and calcium‐binding acid repeat protein, were identified in F . alocis . During the invasion of H e L a cells, there was increased expression of several of the genes encoding these proteins in the potentially more virulent F . alocis D‐62D compared to F . alocis ATCC 35896, the type strain. A comparative protein in silico analysis of the proteome revealed more cell wall anchoring proteins in the F . alocis D‐62D compared to F . alocis ATCC 35896. Their expression was enhanced by coinfection with P orphyromonas gingivalis . Taken together, the variation in the pathogenic potential of the F . alocis strains may be related to the differential expression of several putative virulence factors.