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Metabolic and proteomic adaptation of L actobacillus rhamnosus strains during growth under cheese‐like environmental conditions compared to de M an, R ogosa, and S harpe medium
Author(s) -
Bove Claudio Giorgio,
Angelis Maria De,
Gatti Monica,
Calasso Maria,
Neviani Erasmo,
Gobbetti Marco
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200157
Subject(s) - lactobacillus rhamnosus , biosynthesis , biochemistry , biology , amino acid , lactic acid , food science , bacteria , metabolism , secondary metabolism , metabolic pathway , chemistry , fermentation , enzyme , lactobacillus , genetics
The aim of this study was to demonstrate the metabolic and proteomic adaptation of L actobacillus rhamnosus strains, which were isolated at different stages of P armigiano R eggiano cheese ripening. Compared to de M an, R ogosa, and S harpe ( MRS ) broth, cultivation under cheese‐like conditions (cheese broth, CB ) increased the number of free amino acids used as carbon sources. Compared with growth on MRS or pasteurized and microfiltrated milk, all strains cultivated in CB showed a low synthesis of d,l ‐lactic acid and elevated levels of acetic acid. The proteomic maps of the five representative strains, showing different metabolic traits, were comparatively determined after growth on MRS and CB media. The amount of intracellular and cell‐associated proteins was affected by culture conditions and diversity between strains, depending on their time of isolation. Protein spots showing decreased (62 spots) or increased (59 spot) amounts during growth on CB were identified using MALDI ‐ TOF ‐ MS / MS or LC ‐nano‐ ESI ‐ MS / MS . Compared with cultivation on MRS broth, the L . rhamnosus strains cultivated under cheese‐like conditions had modified amounts of some proteins responsible for protein biosynthesis, nucleotide, and carbohydrate metabolisms, the glycolysis pathway, proteolytic activity, cell wall, and exopolysaccharide biosynthesis, cell regulation, amino acid, and citrate metabolism, oxidation/reduction processes, and stress responses.