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MS ‐based ligand binding assays with speed, sensitivity, and specificity
Author(s) -
Roth Michael J.,
Kim Jaekuk,
Maresh Erica M.,
Plymire Daniel A.,
Corbett John R.,
Zhang Junmei,
Patrie Steven M.
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200151
Subject(s) - chemistry , chromatography , monolayer , ligand (biochemistry) , matrix (chemical analysis) , immunoassay , biochemistry , biology , receptor , antibody , immunology
Immunoassays are widely used in biochemical/clinical laboratories owing to their simplicity, speed, and sensitivity. We combined self‐assembled monolayer‐based immunoassays with MALDI ‐ TOF MS to show that high‐fidelity surface preparations with a novel matrix deposition/crystallization technique permits quantitative analysis of monolayer‐bound antigens at picomolar detection limits. Calibration curves for intact proteins are possible over a broad concentration range and improved specificity of MS ‐immunoassays is highlighted by simultaneous label‐free quantitation of ligand‐bound protein complexes.