Premium
Preparation of sandwich‐structured graphene/mesoporous silica composites with C 8‐modified pore wall for highly efficient selective enrichment of endogenous peptides for mass spectrometry analysis
Author(s) -
Yin Peng,
Wang Yuhua,
Li Yan,
Deng Chunhui,
Zhang Xiangmin,
Yang Pengyuan
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200116
Subject(s) - graphene , mesoporous material , materials science , nanocomposite , mesoporous silica , chemical engineering , adsorption , composite number , specific surface area , coating , surface modification , nanotechnology , chemistry , composite material , organic chemistry , engineering , catalysis
In this study, sandwich‐structured graphene/mesoporous silica composites ( C 8‐modified graphene@m S i O 2 ) were synthesized by coating mesoporous silica onto hydrophilic graphene nanosheets through a surfactant‐mediated cocondensation sol‐gel process. The newly prepared C 8‐modified graphene@m S i O 2 nanocomposites possess unique properties of extended plate‐like morphology, good water dispersibility, highly open pore structure, uniform pore size (2.8 nm), high surface area (632 m 2 /g), and C 8‐modified‐interior pore walls. The unique structure of the C 8‐modified graphene@m S i O 2 composite nanosheets not only provide extended planes with hydrophilic surface that prevents aggregation in solution, but also offer a huge number of C 8‐modified mesopores with high surface area that can ensure an efficient adsorption of peptides through hydrophobic–hydrophobic interaction between C 8‐moified pore walls and target molecules. The obtained C 8‐modified graphene@m S i O 2 materials were utilized for size selectively and specifically enriching peptides in standard peptide mixtures and endogenous peptides in real biological samples (mouse brain tissue).