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Profiling of the human monocytic cell secretome by quantitative label‐free mass spectrometry identifies stimulus‐specific cytokines and proinflammatory proteins
Author(s) -
Groessl M.,
Luksch H.,
RösenWolff A.,
Shevchenko A.,
Gentzel M.
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200108
Subject(s) - proinflammatory cytokine , immune system , secretion , biology , proteomics , microbiology and biotechnology , quantitative proteomics , immunology , inflammation , biochemistry , gene
The immune response to pathogens or injury relies on the concerted release of cytokines and proteins with biological activity important for host protection, host defense, and wound healing. Consequently, the secretome of immune cells provides a promising resource for discovery of specific molecular markers and targets for pharmacological intervention. Here, we employ label‐free MS for unbiased, quantitative profiling of the human monocytic cell secretome under different proinflammatory stimuli. The quantitative secretome profiles reveal the highly stimulus‐dependent cellular response and differential, specific secretion of more than 200 proteins, including important proinflammatory proteins and cytokines.