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Proteomic exploration of the impacts of pomegranate fruit juice on the global gene expression of prostate cancer cell
Author(s) -
Lee SongTay,
Wu YiLing,
Chien LanHsiang,
Chen SzuTing,
Tzeng YuKai,
Wu TingFeng
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200084
Subject(s) - proteomics , prostate cancer , apoptosis , biology , cancer , cancer cell , proteome , cell growth , angiogenesis , cancer research , programmed cell death , signal transduction , cell , microbiology and biotechnology , bioinformatics , biochemistry , gene , genetics
Prostate cancer has been known to be the second highest cause of death in cancer among men. Pomegranate is rich in polyphenols with the potent antioxidant activity and inhibits cell proliferation, invasion, and promotes apoptosis in various cancer cells. This study demonstrated that pomegranate fruit juice could effectively hinder the proliferation of human prostate cancer DU 145 cell. The results of apoptotic analyses implicated that fruit juice might trigger the apoptosis in DU 145 cells via death receptor signaling and mitochondrial damage pathway. In this study, we exploited 2 DE ‐based proteomics to compare nine pairs of the proteome maps collected from untreated and treated DU 145 cells to identify the differentially expressed proteins. Comparative proteomics indicated that 11 proteins were deregulated in affected DU 145 cells with three upregulated and eight downregulated proteins. These dys‐regulated proteins participated in cytoskeletal functions, antiapoptosis, proteasome activity, NF ‐κ B signaling, cancer cell proliferation, invasion, and angiogenesis. Western immunoblotting were implemented to confirm the deregulated proteins and the downstream signaling proteins. The analytical results of this study help to provide insight into the molecular mechanism of inducing prostate cancer cell apoptosis by pomegranate fruit juice and to develop a novel mechanism‐based chemopreventive strategy for prostate cancer.