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Metabolic enzyme diversity in different human thyroid cell lines and their sensitivity to gravitational forces
Author(s) -
Pietsch Jessica,
Sickmann Albert,
Weber Gerhard,
Bauer Johann,
Egli Marcel,
Wildgruber Robert,
Infanger Manfred,
Grimm Daniela
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200070
Subject(s) - glycolysis , enzyme , citric acid cycle , biology , biochemistry , phosphoglycerate kinase , cell culture , cancer cell , thyroid , chemistry , cancer , endocrinology , genetics
Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid cells. Three different types of human thyroid cells were investigated after they had been cultured under equal conditions. MS revealed high quantities of glycolytic enzymes and moderate quantities of citric acid cycle enzymes in malignant FTC‐133 cells with abnormal LDH B‐chains, high quantities of glycolytic enzymes and marginal quantities of citric acid cycle enzymes in normal HTU‐5 cells, and low quantities of glycolytic enzymes and marginal quantities of citrate cycle enzymes in malignant CGTH‐W1 cells with abnormal LDH A‐chains. When an alteration of gene expression activity was challenged physically by removing gravity forces, the concentrations of various glycolytic enzymes were changed in normal and malignant thyroid cells. However, the changes varied among the different cell types. Different cellular alignment of the enzymes could be one reason for the cell type‐specific behavior as demonstrated by histological analysis of alpha‐enolase.