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Identification of intracellular peptides in rat adipose tissue: Insights into insulin resistance
Author(s) -
Berti Denise A.,
Russo Lilian C.,
Castro Leandro M.,
Cruz Lilian,
Gozzo Fábio C.,
Heimann Joel C.,
Lima Fabio B.,
Oliveira Ariclécio C.,
Andreotti Sandra,
Prada Patrícia O.,
Heimann Andrea S.,
Ferro Emer S.
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200051
Subject(s) - adipose tissue , intracellular , insulin resistance , endocrinology , medicine , insulin , glucose uptake , 3t3 l1 , white adipose tissue , biology , chemistry , biochemistry , adipocyte
Intracellular peptides generated by the proteasome and oligopeptidases have been suggested to function in signal transduction and to improve insulin resistance in mice fed a high‐caloric diet. The aim of this study was to identify specific intracellular peptides in the adipose tissue of Wistar rats that could be associated with the physiological and therapeutic control of glucose uptake. Using semiquantitative mass spectrometry and LC/MS/MS analyses, we identified ten peptides in the epididymal adipose tissue of the Wistar rats; three of these peptides were present at increased levels in rats that were fed a high‐caloric Western diet (WD) compared with rats fed a control diet (CD). The results of affinity chromatography suggested that in the cytoplasm of epididymal adipose tissue from either WD or CD rats, distinctive proteins bind to these peptides. However, despite the observed increase in the WD animals, the evaluated peptides increased insulin‐stimulated glucose uptake in 3T3‐L1 adipocytes treated with palmitate. Thus, intracellular peptides from the adipose tissue of Wistar rats can bind to specific proteins and facilitate insulin‐induced glucose uptake in 3T3‐L1 adipocytes.