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LFQ uant: A label‐free fast quantitative analysis tool for high‐resolution LC ‐ MS / MS proteomics data
Author(s) -
Zhang Wei,
Zhang Jiyang,
Xu Changming,
Li Ning,
Liu Hui,
Ma Jie,
Zhu Yunping,
Xie Hongwei
Publication year - 2012
Publication title -
proteomics
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201200017
Subject(s) - proteomics , label free quantification , quantitative proteomics , chromatography , resolution (logic) , computational biology , computer science , chemistry , biology , artificial intelligence , biochemistry , gene
Database searching based methods for label-free quantification aim to reconstruct the peptide extracted ion chromatogram based on the identification information, which can limit the search space and thus make the data processing much faster. The random effect of the MS/MS sampling can be remedied by cross-assignment among different runs. Here, we present a new label-free fast quantitative analysis tool, LFQuant, for high-resolution LC-MS/MS proteomics data based on database searching. It is designed to accept raw data in two common formats (mzXML and Thermo RAW), and database search results from mainstream tools (MASCOT, SEQUEST, and X!Tandem), as input data. LFQuant can handle large-scale label-free data with fractionation such as SDS-PAGE and 2D LC. It is easy to use and provides handy user interfaces for data loading, parameter setting, quantitative analysis, and quantitative data visualization. LFQuant was compared with two common quantification software packages, MaxQuant and IDEAL-Q, on the replication data set and the UPS1 standard data set. The results show that LFQuant performs better than them in terms of both precision and accuracy, and consumes significantly less processing time. LFQuant is freely available under the GNU General Public License v3.0 at http://sourceforge.net/projects/lfquant/.