In this issue

Abstract Mix or match? BOGO (Buy one, get one (free, except in countries where restricted)) The idea of “one free” appeals to most consumers (and scientists, too) but normally only if they need two of the items. In this case it is multiple protein or peptide identifications and small‐scale purification of selected molecules from complex mixtures. Dunham et al. were in need of more efficient methods to process multiple samples, preferably affinity purified. They report here on comparison of four proteins with different binding partners and complexes: COPS5, eIF4A2, RAF1, and MEPCE. COPS5 has been identified as part of a ubiquitin (COP9) signalosome; RAF1 is a serine‐threonine protein kinase, part of the MAP kinase ERK pathway; MEPCE is the 7SK snRNA. Also involved are chaperones, translation initiation factors and RNA processing factors. Each was fractionated classically and multidimensionally (FLAG) and results analyzed for efficiency. And the winner is… pp. 2603–2612Starting small: Phorbol esters as a model breast cancer system So many parameters, so little time. Particularly cell–cell‐based systems. How about those small molecules, like phorbol esters, able to turn cancers on by turning steroid receptors off? (Add the ability to shut off HER‐2 and you have a triple negative breast cancer, one of the toughest breast tumor types.) Dolai et al. report here on controls of a related tumor recently in the news. The bisindolylmaleimide (Bis)‐binding molecules have been identified as protein kinase C inhibitors in the MDA‐MB‐231 model breast cancer system. Gene ontology analysis of the 174 Bis‐stimulated proteins captured revealed 42% ATP and 6% GTP binding and 21% nucleoside‐triphosphatase activity. Novel evidence added glyceraldehyde‐3‐phosphate dehydrogenase, nucleolar RNA helicase‐2, and heterogeneous nuclear ribonucleoprotein M to the list of regulated enzymes. pp. 2683–2692One‐step dance speeds through the cousins When social obligations require that you dance with all of your younger cousins (and your mother does mean all), colluding with the band leader/DJ to “keep 'em short” will save you time and energy and get you to the poker game sooner. Araki et al. have done just that for developing a clinical test for pregnancy‐induced hypertension (PIH). A number of pathological abnormalities have been assigned to PIH, including dysfunction of the placental endothelium and exaggerated systemic inflammatory response. Its cause is clearly multifactorial but, currently, the only diagnostic is monitoring blood pressure. New technology to the rescue! The “peptidome” is defined by the proteomic pattern of peptides and this group has reported major improvements to MALDI/TOF precision and a single step 1‐D electro‐blotting procedure. During the loading of the “BlotChip,” overabundant species, except cousins, can be selectively avoided. pp. 2727–2737