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Omes of somes and cytes and phages Can you rhyme these lines? Bone marrow dendrocytes are turned on by bacterial LPS; macrophages are from mice; endosomes grow from internalized red latex (styrene) beads; latex beads are bait for catching phagosomes. No rhymes? Me neither. But the prose from Duclos et al. is very interesting. In short, internalization of small latex beads shifts the buoyant density of endosomes in sucrose gradients sufficiently for them to be isolated and purified in respectable quantities. Now proteomic analysis tools can be applied to sequential samples of endosomes for reliable developmental analysis of the organelles. Both macrophages from J774 cells and dendrocytes from C57BL6 mice were stimulated to form endosomes and take up the colored latex beads. The macrophage endocytes and dendrocytes exhibited unexpected differences. pp. 854–864A gel is a gel is a tissue section processing tool It never fails to amaze me what new applications researchers find for “old” equipment and procedures, electrophoresis particularly seems to have more than its share of extra lives. Twenty‐some years after its introduction, the semi‐dry Western blot takes up a new career as a front‐end for two dimensional analysis of tissue sections. Zhu et al. demonstrate the utility of electrophoretically driving proteins out of a tissue section and into successive layers of gels while maintaining 2‐D relationships. The technique, termed Layered Electrophoretic Transfer, uses a stack similar to a conventional semi‐dry transfer except that additional layers are introduced into the stack that retain or pass proteins from the sample, depending on their characteristics. The technique is compatible with immunodetection and mass spectrometry. pp. 883–889Sex and the single eye Sex steroids, estradiol and dihydroxytestosterone most commonly, play a major role in the structure and function of ocular tissues. They also have parts in survival of retinal neurodegenerative diseases, but the defense mechanisms are not clearly defined. In this note, D'Anna et al. report that they found three additional sex steroid‐regulated proteins in 5 to 9 spots using 2‐DE coupled to MALDI‐TOF‐MS: Galectin related interfiber protein (GRIFIN, 2 spots), fatty acid binding protein epidermal‐5 (FABP5, 1 spot), and isoforms of αA‐crystallin (CRYAA, multiple spots). One GRIFIN spot showed volume increases even when cells were injected with drug‐free solvent. This behavior suggested a stress response to intraocular injection. CRYAA has characteristics of a chaperonin, possibly including the ability to prevent Alzheimer's amyloid misfolding. Estradiol was more effective at up‐regulation of CRYAA than dihydroxytestosterone. pp. 986–990