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Proteomic analysis of the lungs of mice infected with different pathotypes of H 5 N 1 avian influenza viruses
Author(s) -
Zhao Dongming,
Liang Libin,
Li Yanbing,
Liu Liling,
Guan Yuntao,
Jiang Yongping,
Chen Hualan
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100619
Subject(s) - influenza a virus subtype h5n1 , virology , biology , highly pathogenic , microbiology and biotechnology , virus
The virulence of influenza virus is determined by viral and host factors. Data on the genetic basis of the virulence of H 5 N 1 influenza viruses have increased over the past decade; however, the contributions of host factors to the outcomes of H 5 N 1 infection remain largely unknown. Here, we tested two chicken H 5 N 1 viruses in mice and found that A /chicken/ VN 1214/2007 was nonlethal in mice and only replicated in the lung, whereas A /chicken/ VN 1180/2006 was highly lethal and replicated systemically in mice. To investigate the host response against these two different virus infections, we performed proteomic analysis by using 2 D DIGE on the lung tissues of mice collected on days 1 and 3 postinoculation with different viruses or PBS as a control. Thirty‐nine differentially expressed ( DE ) proteins related to “immune and stimulus response,” “macromolecular biosynthesis and metabolism,” and “cellular component and cytoskeleton” were identified in the virus‐inoculated groups. Moreover, 13 DE proteins were identified between the two virus‐inoculated groups, implying that these proteins may play important roles in the different outcomes of infection with these two viruses. Our data provide important information regarding the host response to mild and lethal H 5 N 1 influenza virus infection.