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Identification of C 6 ‐ceramide‐interacting proteins in D 6 P 2 T S chwannoma cells
Author(s) -
Kota Venkatesh,
Szulc Zdzislaw M.,
Hama Hiroko
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100527
Subject(s) - ceramide , microbiology and biotechnology , lipid signaling , signal transduction , cell cycle , biology , cell signaling , biochemistry , cell , chemistry , apoptosis , receptor
Ceramide is a bioactive molecule involved in numerous cell signaling pathways that are associated with cell cycle control, differentiation, senescence, and apoptosis. Although substantial knowledge about ceramide‐regulated pathways has accumulated in the past decade, molecular mechanisms of ceramide action remain poorly understood, primarily due to limited information about ceramide‐binding proteins. In the present study, we used affinity purification with a synthetic biotin‐conjugated C 6 ‐ceramide analogue and LC‐MS/MS to identify potential ceramide‐interacting proteins in D 6 P 2 T S chwannoma cells. The purification resulted in identification of 97 unique proteins. The identified proteins are involved in various cellular processes, including apoptosis, cellular stress, cell cycle, cell differentiation, signaling, transcription, translation, protein biogenesis, metabolism, and transport.