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Cell surface proteome of the marine planctomycete R hodopirellula baltica
Author(s) -
Voigt Birgit,
Hieu Cao Xuan,
Hempel Kristina,
Becher Dörte,
Schlüter Rabea,
Teeling Hanno,
Glöckner Frank Oliver,
Amann Rudolf,
Hecker Michael,
Schweder Thomas
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100512
Subject(s) - proteome , biology , enzyme , biochemistry , microbiology and biotechnology , chemistry
The surface proteome (surfaceome) of the marine planctomycete R hodopirellula baltica SH 1 T was studied using a biotinylation and a proteinase K approach combined with SDS ‐ PAGE and mass spectrometry. 52 of the proteins identified in both approaches could be assigned to the group of potential surface proteins. Among them are some high molecular weight proteins, potentially involved in cell–cell attachment, that contain domains shown before to be typical for surface proteins like cadherin/dockerin domains, a bacterial adhesion domain or the fasciclin domain. The identification of proteins with enzymatic functions in the R . baltica surfaceome provides further clues for the suggestion that some degradative enzymes may be anchored onto the cell surface. YTV proteins, which have been earlier supposed to be components of the proteinaceous cell wall of R . baltica , were detected in the surface proteome. Additionally, 8 proteins with a novel protein structure combining a conserved type IV pilin/ N ‐methylation domain and a planctomycete‐typical DUF 1559 domain were identified.