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A proteogenomic approach to map the proteome of an unsequenced pathogen – Leishmania donovani
Author(s) -
Pawar Harsh,
Sahasrabuddhe Nandini A.,
Renuse Santosh,
Keerthikumar Shivakumar,
Sharma Jyoti,
Kumar Ghantasala. S. Sameer,
Venugopal Abhilash,
Sekhar Nirujogi Raja,
Kelkar Dhanashree S.,
Nemade Harshal,
Khobragade Sweta N.,
Muthusamy Babylakshmi,
Kandasamy Kumaran,
Harsha H. C.,
Chaerkady Raghothama,
Patole Milind S.,
Pandey Akhilesh
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100505
Subject(s) - amastigote , leishmania donovani , biology , proteome , leishmania infantum , proteogenomics , visceral leishmaniasis , genome , leishmania , leishmaniasis , proteomics , microbiology and biotechnology , computational biology , parasite hosting , gene , genetics , genomics , world wide web , computer science
Visceral leishmaniasis or kala azar is the most severe form of leishmaniasis and is caused by the protozoan parasite Leishmania donovani . There is no published report on L. donovani genome sequence available till date, although the genome sequences of three related Leishmania species are already available. Thus, we took a proteogenomic approach to identify proteins from two different life stages of L. donovani . From our analysis of the promastigote (insect) and amastigote (human) stages of L. donovani , we identified a total of 22322 unique peptides from a homology‐based search against proteins from three Leishmania species. These peptides were assigned to 3711 proteins in L. infantum, 3287 proteins in L. major , and 2433 proteins in L. braziliensis . Of the 3711 L. donovani proteins that were identified, the expression of 1387 proteins was detectable in both life stages of the parasite, while 901 and 1423 proteins were identified only in promastigotes and amastigotes life stages, respectively. In addition, we also identified 13 N‐terminally and one C‐terminally extended proteins based on the proteomic data search against the six‐frame translated genome of the three related Leishmania species. Here, we report results from proteomic profiling of L. donovani , an organism with an unsequenced genome.