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Proteomic analysis of Taenia solium metacestode excretion–secretion proteins
Author(s) -
Victor Bjorn,
Kanobana Kirezi,
Gabriël Sarah,
Polman Katja,
Deckers Nynke,
Dorny Pierre,
Deelder André M.,
Palmblad Magnus
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100496
Subject(s) - metacestode , taenia solium , neurocysticercosis , proteome , biology , cysticercosis , secretion , parasitic disease , excretion , helminths , immunology , disease , medicine , zoology , biochemistry , pathology , cestoda
The metacestode larval stage of Taenia solium is the causal agent of a zoonotic disease called cysticercosis. The disease has an important impact on pork trade (due to porcine cysticercosis) and public health (due to human neurocysticercosis). In order to improve the current diagnostic tools and to get a better understanding of the interaction between T. solium metacestodes and their host, there is a need for more information about the proteins that are released by the parasite. In this study, we used protein sequences from different helminths, 1DE, reversed‐phase LC, and MS/MS to analyze the excretion–secretion proteins produced by T. solium metacestodes from infected pigs. This is the first report of the T. solium metacestode excretion–secretion proteome. We report 76 proteins including 27 already described T. solium proteins, 17 host proteins and 32 proteins likely to be of T. solium origin, but identified using sequences from other helminths.