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A quantitative proteomic analysis of lung epithelial (A549) cells infected with 2009 pandemic influenza A virus using stable isotope labelling with amino acids in cell culture
Author(s) -
Dove Brian K.,
Surtees Rebecca,
Bean Thomas J.H.,
Munday Diane,
Wise Helen M.,
Digard Paul,
Carroll Miles W.,
Ajuh Paul,
Barr John N.,
Hiscox Julian A.
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100470
Subject(s) - labelling , stable isotope labeling by amino acids in cell culture , a549 cell , cell culture , virology , virus , influenza a virus , biology , pandemic , pandemic influenza , proteomics , covid-19 , biochemistry , medicine , pathology , gene , genetics , disease , infectious disease (medical specialty)
Influenza A virus is one of the world's major uncontrolled pathogens, causing seasonal epidemics as well as global pandemics. This was evidenced by the recent emergence and now prevalence of the 2009 swine origin pandemic H1N1 influenza A virus. In this study, quantitative proteomics using stable isotope labelling with amino acids in cell culture was used to investigate the changes in the host cell proteome in cells infected with pandemic H1N1 influenza A virus. The study was conducted in A549 cells that retain properties similar to alveolar cells. Several global pathways were affected, including cell cycle regulation and lipid metabolism, and these could be correlated with recent microarray analyses of cells infected with influenza A virus. Taken together, both quantitative proteomics and transcriptomic approaches can be used to identify potential cellular proteins whose functions in the virus life cycle could be targeted for chemotherapeutic intervention