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Two‐dimensional gel‐based alkaline proteome of the probiotic bacterium L actobacillus acidophilus NCFM
Author(s) -
Majumder Avishek,
Cai Liyang,
Ejby Morten,
Schmidt Bjarne G.,
Lahtinen Sampo J.,
Jacobsen Susanne,
Svensson Birte
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100455
Subject(s) - proteome , bacteria , probiotic , proteomics , lactic acid , biochemistry , lactobacillus acidophilus , chemistry , biology , food science , gene , genetics
L actobacillus acidophilus NCFM ( NCFM ) is a well‐documented probiotic bacterium isolated from human gut. Detailed 2D gel‐based NCFM proteomics addressed the so‐called alkaline range, i.e., pH 6–11. Proteins were identified in 150 of the 202 spots picked from the C oomassie B rilliant B lue stained 2D gel using MALDI ‐ TOF ‐ MS . The 102 unique gene products among the 150 protein identifications were assigned to different functional categories, and evaluated by considering a calculated distribution of abundance as well as grand average of hydrophobicity values. None of the very few available lactic acid bacteria proteome reference maps included the range of p I >7.0. The present report of such data on the proteome of NCFM fundamentally complements current knowledge on protein profiles limited to the acid and neutral pH range.