Premium
Identification of centromeric and telomeric DNA ‐binding proteins in rice
Author(s) -
He Qi,
Chen Lei,
Xu Yu,
Yu Weichang
Publication year - 2013
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100416
Subject(s) - telomere binding protein , centromere , telomere , biology , dna , dna binding protein , genetics , eukaryotic chromosome fine structure , microbiology and biotechnology , chromosome , gene , transcription factor
Centromeres and telomeres are DNA /protein complexes and essential functional components of eukaryotic chromosomes. Previous studies have shown that rice centromeres and telomeres are occupied by Cent O ( rice centromere satellite DNA ) satellite and G ‐rich telomere repeats, respectively. However, the protein components are not fully understood. DNA‐binding proteins associated with centromeric or telomeric DNA s will most likely be important for the understanding of centromere and telomere structure and functions. To capture DNA ‐specific binding proteins, affinity pull‐down technique was applied in this study to isolate rice centromeric and telomeric DNA ‐binding proteins. Fifty‐five proteins were identified for their binding affinity to rice Cent O repeat, and 80 proteins were identified for their binding to telomere repeat. One Cent O ‐binding protein, O s02g0288200, was demonstrated to bind to Cent O specifically by in vitro assay. A conserved domain, DUF 573 with unknown functions was identified in this protein, and proven to be responsible for the specific binding to Cent O in vitro. Four proteins identified as telomere DNA‐binding proteins in this study were reported by different groups previously. These results demonstrate that DNA affinity pull‐down technique is effective in the isolation of sequence‐specific binding proteins and will be applicable in future studies of centromere and telomere proteins.