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Proteomic analysis of rat microglia establishes a high‐confidence reference data set of over 3000 proteins
Author(s) -
BellTemin Harris,
Barber David S.,
Zhang Ping,
Liu Bin,
Stevens Stanley M.
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100398
Subject(s) - proteome , orbitrap , microglia , chemistry , fractionation , computational biology , tandem mass spectrometry , proteomics , mass spectrometry , function (biology) , chromatography , biochemistry , biology , microbiology and biotechnology , immunology , inflammation , gene
Highly aggressively proliferating immortalized (HAPI) microglial cells have been used as an in vitro model for investigating key microglial functions including inflammatory, neurotoxic, and phagocytic activities. Through the use of offline strong cation‐exchange fractionation followed by inline reversed‐phase chromatographic separation and tandem mass spectrometric analysis on a hybrid linear ion trap‐Orbitrap instrument, the HAPI microglial proteome was characterized to a depth of 3006 unique protein groups. Upon bioinformatic analysis of the HAPI proteome data set, enrichment was observed for processes relevant to microglial function including those associated with immune system response. This study marks the most comprehensive reference data set generated to date for the rat microglial proteome.