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Whole‐cell proteome reference maps of an extreme thermophile, T hermus thermophilus HB 8
Author(s) -
Kim Kwang,
Okanishi Hiroki,
Masui Ryoji,
Harada Akira,
Ueyama Norikazu,
Kuramitsu Seiki
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100375
Subject(s) - proteome , biochemistry , thermus thermophilus , thermophile , metabolism , biology , enzyme , chemistry , gene , escherichia coli
T hermus thermophilus HB 8 is a model microorganism for industrial applications because of its thermophilic enzymes, and for basic bacteriology to understand the coordination of the biological functions of the genome‐encoded enzymes at the cellular level. Here, we present 2 DE reference maps of T . thermophilus HB 8 in the pH ranges 4–7 and 6–11 obtained with whole‐cell lysates. PMF analysis using MALDI ‐ TOF ‐ MS and MS / MS analysis using nano‐scale LC and quadrupole TOF ‐ MS identified 258 different proteins among the 306 protein spots on 2 DE gels. Functional classification indicated that 56%, 16%, and 14% of the identified proteins were related to metabolism, genetic information process, and cellular process, respectively. Detailed classification of the metabolism‐related proteins suggested that during the exponential phase, amino acid and carbohydrate metabolism are major metabolic processes, whereas nucleotide and lipid metabolism are minor ones. On the other hand, volume quantification analysis revealed that proteins involved in the translational process, nucleotide metabolism, and central carbon metabolism were most abundantly expressed in the exponential phase.