The OASIS ® HLB μ E lution plate as a one‐step platform for manual high‐throughput in‐gel digestion of proteins and peptide desalting

Separation or prefractionation of proteins by gel electrophoresis, followed by in‐gel proteolytic digest and analysis by MS is a typical standard application in proteomics. For many laboratories, it is not cost‐effective to use a robot with all its drawbacks. On the other hand, manual digest is time consuming and not free of error if many samples are processed at the same time. The OASIS ® HLB μ E lution plate allows the handling of 96 samples for protein in‐gel digest and peptide desalting in a semiautomatic way. Using multichannel pipettes, solutions are added quickly, and with the use of the positive pressure‐96 stand all solutions are removed simultaneously. In this article, a complete and detailed protocol for the use of the OASIS ® HLB μ E lution plate is introduced and its effectiveness is shown with 2 D ‐ and 1 D ‐gel samples.