Premium
Evaluation of SCO 1 deletion on S accharomyces cerevisiae metabolism through a proteomic approach
Author(s) -
Gamberi Tania,
Puglia Michele,
Bianchi Laura,
Gimigliano Anna,
Landi Claudia,
Magherini Francesca,
Guidi Francesca,
Ranaldi Francesco,
Armini Alessandro,
Cipriano Maria,
Gagliardi Assunta,
Modesti Alessandra,
Bini Luca
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100285
Subject(s) - mutant , saccharomyces cerevisiae , biochemistry , alcohol dehydrogenase , pyruvate decarboxylase , biology , yeast , cytochrome c oxidase , dehydrogenase , glycolysis , metabolism , enzyme , gene
The S accharomyces cerevisiae gene SCO 1 has been shown to play an essential role in copper delivery to cytochrome c oxidase. Biochemical studies demonstrated specific transfer of copper from C ox17p to S co1p, and physical interactions between the S co1p and C ox2p. Deletion of SCO 1 yeast gene results in a respiratory deficient phenotype. This study aims to gain a more detailed insight on the effects of SCO 1 deletion on S . cerevisiae metabolism. We compared, using a proteomic approach, the protein pattern of SCO 1 null mutant strain and wild‐type BY 4741 strain grown on fermentable and on nonfermentable carbon sources. The analysis showed that on nonfermentable medium, the SCO 1 mutant displayed a protein profile similar to that of actively fermenting yeast cells. Indeed, on 3% glycerol, this mutant displayed an increase of some glycolytic and fermentative enzymes such as glyceraldehyde‐3‐phosphate dehydrogenase 1, enolase 2, pyruvate decarboxylase 1, and alcohol dehydrogenase 1. These data were supported by immunoblotting and enzyme activity assay. Moreover, the ethanol assay and the oxygen consumption measurement demonstrated a fermentative activity in SCO 1 mutant on respiratory medium. Our results suggest that on nonfermentable carbon source, the lack of S co1p causes a metabolic shift from respiration to fermentation.