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Proteomic analysis of the effects of baicalein on colorectal cancer cells
Author(s) -
Huang WenShih,
Kuo YiHung,
Chin ChihChien,
Wang JengYi,
Yu HongRen,
Sheen JiunnMing,
Tung ShuiYi,
Shen ChienHeng,
Chen TeChuan,
Sung MeiLan,
Liang HweyFang,
Kuo HsingChun
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100270
Subject(s) - baicalein , cell growth , chemistry , colorectal cancer , gene knockdown , cell , cancer cell , apoptosis , cancer research , pharmacology , cancer , microbiology and biotechnology , biology , biochemistry , genetics
Baicalein is the flavonoids with multiple pharmacological activities. The aim of our study was to investigate the effects of baicalein on colorectal cancer (CRC) and to recognize the targets of baicalein treatment. To better understand baicalein's target, proteomic approaches were used to purify and identify the protein substrates using 2D difference gel electrophoresis (2D SDS‐PAGE) to elucidate proteins differential display. Results from this study investigate that baicalein treatment of CRC cells results in reduced cell proliferation. As a result, differential protein displays between baicalein‐treated and untreated CRC were determined and validated. There were 11 differentially expressed proteins between baicalein‐treated and untreated CRC. Furthermore, we demonstrate that baicalein inhibits cancer cell proliferation and reduced reactive oxygen species (ROS) by up‐regulating the levels of peroxiredoxin‐6 (PRDX6). Knockdown of PRDX6 in baicalein‐treated CRC cells by specific small interfering RNA resulted in ROS production and proliferation, opposite of the baicalein treatment scenario as indicated by cell cycle distribution. These results illustrate that baicalein up‐regulates the expression of PRDX6, which attenuates the generation of ROS and inhibits the growth of CRC cells, whereas baicalein treatment have no effect on normal epithelial cells.