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Proteomic analysis of the microenvironment of developing oocytes
Author(s) -
Twigt John,
SteegersTheunissen Regine P.,
Bezstarosti Karel,
Demmers Jeroen A. A.
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100240
Subject(s) - proteome , oocyte , follicular fluid , fractionation , proteomics , immune system , biology , microbiology and biotechnology , chemistry , computational biology , biochemistry , immunology , chromatography , embryo , gene
We utilized a setup based on extensive pre‐fractionation of proteolytic peptides and nanoflow reversed‐phase LC ‐ MS / MS to identify the (sub)proteome of human follicular fluid ( FF ). In this in‐depth screen, 246 specific proteins were identified, the majority of which are involved in coagulation‐ and immune‐response pathways. Our aim is to define a set of FF protein markers, which could predict oocyte quality.