Premium
Roles of interferon‐gamma and its target genes in schizophrenia: Proteomics‐based reverse genetics from mouse to human
Author(s) -
Kim HakJae,
Eom ChiYong,
Kwon Joseph,
Joo Jaesoon,
Lee Sujeong,
Nah SeongSu,
Kim IlChul,
Jang IkSoon,
Chung YoungHo,
Kim Seung Il,
Chung JooHo,
Choi JongSoon
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100184
Subject(s) - biology , single nucleotide polymorphism , gene , microbiology and biotechnology , snp , downregulation and upregulation , population , proteomics , genetics , genotype , medicine , environmental health
A decreased production of interferon gamma ( IFNG ) has been observed in acute schizophrenia. In order to explore the possible relationship between IFNG and schizophrenia, we attempted to analyze the differentially expressed proteins in the brains of interferon‐gamma knockout ( I fng ‐ KO ) mice. Five upregulated and five downregulated proteins were identified with 2D gels and MALDI ‐ TOF / TOF MS analyses in I fng ‐ KO mouse brain. Of the identified proteins, we focused on creatine kinase brain ( CKB ) and triose phosphate isomerase 1 ( TPI 1). Consistent with the proteomic data, reverse transcriptase‐mediated PCR , immunoblotting, and immunohistochemistry analyses confirmed that the levels of gene expressions of C kb and T pi1 were downregulated and upregulated, respectively. When we analyzed the genetic polymorphisms of the single nucleotide polymorphisms ( SNP s) of their human orthologous genes in a Korean population, the promoter SNP s of CKB and TPI 1 were weakly associated with schizophrenia. In addition, IFNG polymorphisms were associated with schizophrenia. These results suggest that IFNG and proteins affected by IFNG may play a role in the pathogenesis of schizophrenia.