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Proteomic profiling of T ectona grandis L . leaf
Author(s) -
Quiala Elisa,
Cañal María Jesús,
Rodríguez Roberto,
Yagüe Norma,
Chávez Maité,
Barbón Raúl,
Valledor Luis
Publication year - 2012
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100183
Subject(s) - proteome , biology , spots , proteomics , botany , protein purification , biochemistry , gene
T ectona grandis L . (teak) is one of the premier hardwood timbers in the world, ranking at present in the top five tropical hardwood species in terms of worldwide plantation area. Characterization of the proteins present in teak leaves will provide a basis for the development of new tools aimed at assisting tree selection, the monitoring of plant propagation, and the certification of clonal and phenotypic identities. In this paper, we describe the extraction, separation, and identification of leaf proteins from T . grandis using a TCA /acetone protocol, 2DE, and MALDI‐TOF. After TCA /acetone protein extraction of leaves, 998 well‐resolved spots were detected in Coomassie‐stained gels within the 10–114 k D a relative molecular mass ( M r ) range at a p H ranging from 3 to 11. A total of 120 spots were digested and subjected to MS . Of these, 100 nonredundant protein species were successfully identified. Functional classification of the identified proteins revealed that proteins involved in photosynthesis, protein translation, and energy production were the most abundant. This work is the first high‐throughput attempt to study the T . grandis leaf proteome and represents a stepping stone for further differential expression proteomic studies related to growth, development, biomass production, and culture‐associated physiological responses.