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Sites of ubiquitin attachment in Saccharomyces cerevisiae
Author(s) -
Starita Lea M.,
Lo Russell S.,
Eng Jimmy K.,
von Haller Priska D.,
Fields Stanley
Publication year - 2011
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/pmic.201100166
Subject(s) - saccharomyces cerevisiae , ubiquitin , yeast , biochemistry , chemistry , peptide , ubiquitin conjugating enzyme , mass spectrometry , ubiquitin ligase , chromatography , gene
Sites of ubiquitin modification have been identified by mass spectrometry based on the increase in molecular mass of a tryptic peptide carrying two additional glycine residues from the ubiquitin moiety. However, such peptides with GG shifts have been difficult to discover. We identify 870 unique sites of ubiquitin attachment on 438 different proteins of the yeast Saccharomyces cerevisiae .